| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.046104 |
| Chromosome: | chromosome 3 |
| Location: | 8255146 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199750 | (1 of 1) PTHR12231:SF147 - LACHESIN-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCTTTGTTAAAACCACACACACACACAGAGACGAGGCTGAATGCCAAA |
| Internal bar code: | AATATTGAAATTGTTGTCGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 850 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTTGAGAGCACTGGACT |
| Suggested primer 2: | CATAATGCAAGTCCCGCAGC |