| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.046109 |
| Chromosome: | chromosome 9 |
| Location: | 5575863 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409600 | FAP411 | Flagellar Associated Protein 411 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTTACCTCGCGGCATTCCTCTGCCGCGCGGTTGCGTTAGTAGTGATGC |
| Internal bar code: | AGTCTTGCGTTTCGGGGTGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1942 |
| LEAP-Seq percent confirming: | 37.5 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGATCGCTTTTCCCATGCA |
| Suggested primer 2: | ATTCTGCAACTCACCCCTCG |