| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.046149 |
| Chromosome: | chromosome 2 |
| Location: | 6551518 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389150 | FAP93 | Flagellar Associated Protein 93 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTCTGGCATGCTCCGGCCATGCCCTTCACCCCGGCGTCACCTCTACCC |
| Internal bar code: | CTTATGAACACATGTACCCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3583 |
| LEAP-Seq percent confirming: | 73.6842 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCCATCCATGAACGGGGG |
| Suggested primer 2: | GTCATTTCCATCTGCCGTGC |