Insertion junction: CLIP2.046156_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.046156
Chromosome:chromosome 2
Location:2394241
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g091100 RPL15 (1 of 1) K02877 - large subunit ribosomal protein L15e (RP-L15e, RPL15); Cytosolic 80S ribosomal protein L15 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCGTGGGCGCATCAGTTGCGTCCGGCTCTAAATTGATTTGTGTCCTAGC
Internal bar code:TTGCTAGACGATCCGCCTCTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2132
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTGGATGATACGAGTGGT
Suggested primer 2:ATCCAGCGACAACTCACGTT