| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.046159 |
| Chromosome: | chromosome 11 |
| Location: | 1439504 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g469500 | FXL6 | FixL-like PAS domain protein; (1 of 12) 2.7.13.3 - Histidine kinase / Protein kinase (histidine) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAATCCTTACGGCTCCACATTCCGTACATGTCCGTGGCCAGCTCCCGCA |
| Internal bar code: | TGTCACTTCTTGTGCCAGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5643 |
| LEAP-Seq percent confirming: | 99.0566 |
| LEAP-Seq n confirming: | 105 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 106 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCCTCGCTTACACGGTT |
| Suggested primer 2: | GGGGTTATGAGAGAGGGGGT |