Insertion junction: CLIP2.046170_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.046170
Chromosome:chromosome 14
Location:2447187
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g623750 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCTGTGTTTCATCCACTCCCGTGCTTGATTTCCAGTCAGTATCTAATG
Internal bar code:TCACCCTTTCTTAGAGCATAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2247
LEAP-Seq percent confirming:94.1176
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:ACCACCACAAACCACGACTT
Suggested primer 2:TAGACAAAACGTTTGGCGCG