| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.046173 |
| Chromosome: | chromosome 2 |
| Location: | 6992239 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389504 | (1 of 10) PF13578 - Methyltransferase domain (Methyltransf_24) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAGGTAGTACTTCTGACACGACGGCCACGTTTTTAGCCTGTACATGCG |
| Internal bar code: | CTTTGTAAGTACAGTGAAGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3106 |
| LEAP-Seq percent confirming: | 56.6667 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGGCCCCACTACGTATTC |
| Suggested primer 2: | TGAAGTTGCGCAGAAACACG |