| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.046175 |
| Chromosome: | chromosome 12 |
| Location: | 9286117 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g542500 | STM6,MOC1 | Mitochondrial transcription termination factor; (1 of 10) PF02536 - mTERF (mTERF) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGCGGTCCAGGGAGAGCTGGAAGGCCTGAGCGGCAGAGGGGGAGCGGC |
| Internal bar code: | ATTATGTGAATTGCAAATCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 858 |
| LEAP-Seq percent confirming: | 3.7037 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTGGGGTATCGCCGAACG |
| Suggested primer 2: | AGATGCTGAGGTGGTGGTTG |