| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.046184 |
| Chromosome: | chromosome 2 |
| Location: | 915015 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g800175 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTTCAATGCTGTGCCTGCCCTCCCCCCGCCAATGCCTCGAAACACAGA |
| Internal bar code: | GGAACGGCAGTTTGTGAATTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2456 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTCTCTCGGGGAGTTCCA |
| Suggested primer 2: | CTGTTGTTCAGCTGTGGTGC |