Insertion junction: CLIP2.046199_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.046199
Chromosome:chromosome 9
Location:2593614
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g801017 (1 of 3) K14007 - protein transport protein SEC24 (SEC24) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTTACTCATTCCATCATGGGCGGCATCATCGCCTCTCCCACACACCCC
Internal bar code:ACGCTTAGATCCTATCAACGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:109
LEAP-Seq percent confirming:29.2683
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:29
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCCCAAACCTCCAATCCC
Suggested primer 2:TGGGTTAAGGACGTCACGTG