Insertion junction: CLIP2.046219_4

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.046219
Chromosome:chromosome 12
Location:4965471
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCATGCGCCCCGAAACCCCCACCCCGTCGCCACCCACCAACTGTCGCTC
Internal bar code:CTTCTGTAGGGTTCTTCTGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:3862
LEAP-Seq percent confirming:87.8049
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:CACAAAATGCACCCACCTGG
Suggested primer 2:CGTGCCATTTGTAGGATGCG