| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.046221 |
| Chromosome: | chromosome 8 |
| Location: | 3385242 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g377850 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCTGGCCGACCGCGGCTGGCTGATCCACCCGCGGCAGAACCGCCTGGC |
| Internal bar code: | ATTATATTAATGACAGTTTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 82 |
| LEAP-Seq percent confirming: | 16.6667 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGTCCCTCCAGAAGGCCA |
| Suggested primer 2: | GCGCGGTATGTGTGCTAAAG |