| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.046233 |
| Chromosome: | chromosome 7 |
| Location: | 187924 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313400 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGACACGCTGCGGACTGCCGCGGCCAAGAACACGCGCGTGAGCTTGGCG |
| Internal bar code: | GGTCCCTATAACCATGTAAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3973 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTTAACACACGCCGGCTG |
| Suggested primer 2: | AATGGACAGCAGCGGATTGA |