Insertion junction: CLIP2.046236_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.046236
Chromosome:chromosome 3
Location:7644485
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g204300 (1 of 41) IPR000719//IPR011009 - Protein kinase domain // Protein kinase-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGACGTCTCGCCACGCACCCCGCGACCCCAACGCCCACCACCCCCGCC
Internal bar code:CAATGATTATATATCGTTTTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:191
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GACTACGCGCTTCATGCAAG
Suggested primer 2:TCAAAGCCTGTCGCATTCCT