Insertion junction: CLIP2.046238_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.046238
Chromosome:chromosome 4
Location:1309197
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g214650 BGS5,GSL1,GTR19 Glucan synthase-like 1; (1 of 7) 2.4.1.34 - 1,3-beta-glucan synthase / UDP-glucose-1,3-beta-D-glucan glucosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAAACCCATACAAAAAGCAGCATCCAAATAAACCCATGCAAGAAGCACA
Internal bar code:CAAGTCTCTAGATTGTGAGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:373
LEAP-Seq percent confirming:16.6667
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:CTCTCCCAATCCCTCTCCCA
Suggested primer 2:CGCACCTCCATTGTCTCCTT