Insertion junction: CLIP2.046249_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.046249
Chromosome:chromosome 9
Location:376451
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre18.g749947 FAP21 Flagellar Associated Protein 21; (1 of 1) PTHR24343//PTHR24343:SF158 - SERINE/THREONINE KINASE // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCGGCTCGCGCTGGGCACTGCGCAGACGTGTGGACACGGCGATGACGA
Internal bar code:TGTATCGGATGGCATGGTTCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:913
LEAP-Seq percent confirming:3.125
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:AATCCCTCCGACGGACAAAC
Suggested primer 2:GACCCCAGAGTAGGAGAGCA