| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.046251 |
| Chromosome: | chromosome 9 |
| Location: | 363727 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g750047 | FMG1,FMG1-1,FMG1A,FMG1-A | Flagella Membrane Glycoprotein 1A | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCAACGACCGCCTCAACGTGCGCTCCTCCAACACCATCATGACCGTGC |
| Internal bar code: | GCCAAAGGGTTCGCCAACGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2583 |
| LEAP-Seq percent confirming: | 63.6364 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGATGGTGCAGCTGGAGAA |
| Suggested primer 2: | TGTGGTCCAGCAGTGATGAC |