Insertion junction: CLIP2.046255_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.046255
Chromosome:chromosome 8
Location:2602440
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g373200 FXL7 FixL-like PAS domain protein; (1 of 11) PF13426 - PAS domain (PAS_9) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGCCCTGCTGCAGCCCCTCGTACAGGTCCGCGCCCGCCACCGCCGCCA
Internal bar code:TTTCGAGAGATTTTGCTAAATA

Confirmation - LEAP-Seq

LEAP-Seq distance:2429
LEAP-Seq percent confirming:53.3333
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTAACGTCATCTGCAAGC
Suggested primer 2:AATGAAGTAGCCGCACACCA