Insertion junction: CLIP2.046258_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.046258
Chromosome:chromosome 6
Location:3796959
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278173 OPR59 OctotricoPeptide Repeat protein 59; (1 of 1) K08515 - vesicle-associated membrane protein 7 (VAMP7) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACCACTGGCCACCTCCACCTGCGGGGCGCGGCCGCCAGGCCGCATGCA
Internal bar code:AGCAGGCTGTCCGCCTACTCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2511
LEAP-Seq percent confirming:36.9048
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:53
LEAP-Seq n unique pos:84

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTGTGTCAGGGAAGGGTC
Suggested primer 2:GCAAGTCCACAACACGACAC