| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.046259 |
| Chromosome: | chromosome 13 |
| Location: | 2641671 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g581200 | (1 of 1) PTHR12241//PTHR12241:SF1 - TUBULIN TYROSINE LIGASE-RELATED // TUBULIN--TYROSINE LIGASE PBY1-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGATGGCTGCTCGGACCTCCCACTGCAGCGGCAGCCTTTTTCTTGTG |
| Internal bar code: | GAAGTATGTATAGTAGTAGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1003 |
| LEAP-Seq percent confirming: | 64.2857 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGATGTCATGCAAGCGTCG |
| Suggested primer 2: | GAACTGTCACAGCTGACCGA |