| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.046267 |
| Chromosome: | chromosome 3 |
| Location: | 3319385 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g165900 | PGM17 | (1 of 3) 3.1.3.46 - Fructose-2,6-bisphosphate 2-phosphatase / Fructose-2,6-bisphosphatase; Putative phosphoglycerate mutase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTATGTACACCCGCCCGCCCGACCCGCAGGCTCGGGAGGCGGGGCAGC |
| Internal bar code: | CACTGTCGCTCGTCCTCGCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1365 |
| LEAP-Seq percent confirming: | 36.3636 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAACGCGCAACATGACCAG |
| Suggested primer 2: | CGAAATCGCTTGGAGCATGG |