Insertion junction: CLIP2.046270_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.046270
Chromosome:chromosome 10
Location:635572
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g421900 SRR27 Scavenger receptor cysteine rich (SRCR) protein; (1 of 5) 1.4.3.13 - Protein-lysine 6-oxidase / Lysyl oxidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGGGAGGGAGGAAGCGAGCGGAGGGGAGGGCACGGGAGGGGAGGGGAA
Internal bar code:GCATGGGAGGTAGCGCGTGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:3309
LEAP-Seq percent confirming:98.6111
LEAP-Seq n confirming:71
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:72

Suggested primers for confirmation by PCR

Suggested primer 1:AGTCGCATCTGCAACTGTCA
Suggested primer 2:GAGCGAGAGTCCGAAGCTAC