Insertion junction: CLIP2.046290_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.046290
Chromosome:chromosome 5
Location:2619648
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g239900 ARS15 Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGCAACCGCATCCACACCTTCCCCCCTGGCTCCCTACAGTGTGACCAAC
Internal bar code:GAAAGGACCTCAAAATAAGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2975
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:CCGAACCTTGCATATGCGTG
Suggested primer 2:ATTGGTGGTGGGTTTTGGGT