| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.046302 |
| Chromosome: | chromosome 12 |
| Location: | 3864501 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515150 | (1 of 1) K17784 - mitochondrial inner membrane organizing system protein 1 (MINOS1, MOS1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTGCAGGGGCTGCAGCAGCCTGCCCCAGCCTAGCAGACCGGAACGCG |
| Internal bar code: | TGCTATCGCTCCTGCGTAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1308 |
| LEAP-Seq percent confirming: | 92.5926 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGGTCTTCAGCAGCTCC |
| Suggested primer 2: | CATTTTGTGCCCCCAACAGG |