Insertion junction: CLIP2.046305_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.046305
Chromosome:chromosome 2
Location:6009824
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g118650 PHC47 Pherophorin-chlamydomonas homolog 47; (1 of 71) PF12499 - Pherophorin (DUF3707) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGGCAGCCTGCGGTTGGGAGCATGTGGAATCAGTCGATACCGTCTCAT
Internal bar code:ACTTATATTCGGTCCTAATTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:653
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:AATGTACGTGTCCTGCGTGT
Suggested primer 2:GGCCTTACACCACATGCTCT