| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.046308 |
| Chromosome: | chromosome 12 |
| Location: | 221776 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g484800 | (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTAATAGTGTGCTTCTACGTCTACGTGCAGCCCCTTCCACCCCTCCCG |
| Internal bar code: | CGGTCTAAGCCTATTCGTAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2060 |
| LEAP-Seq percent confirming: | 91.3043 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGCACCGAACATGTCCAT |
| Suggested primer 2: | GCTGGAACTGAGCCATGTCT |