Insertion junction: CLIP2.046326_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.046326
Chromosome:plastome
Location:16487
Confidence (%):80
Locus systematic id Locus common name Defline Feature
CreCp.g802269 ChreCp007,clpP,2717015 (1 of 9) 3.4.21.92 - Endopeptidase Clp / Protease Ti; ATP-dependent Clp protease proteolytic subunit CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTACTTCATTTGTTGCAATTTCATCAGCTAAACCATAATAAATTGTTT
Internal bar code:GATTTAGCGTGAGACATGGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:220
LEAP-Seq percent confirming:18.1818
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TGGCGGTTCTGTAGGTAATGG
Suggested primer 2:GGAAACCTGCGTTCTACCGA