Insertion junction: CLIP2.046332_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.046332
Chromosome:chromosome 2
Location:5188383
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g112100 C1a-86,FAP101 (1 of 31) IPR003590 - Leucine-rich repeat, ribonuclease inhibitor subtype; Flagellar Associated Protein 101 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACAGTGCTTAAGCAATAAGCATGTTGCGACACCGCCGTGTTTAAGTCAC
Internal bar code:AGCTTTTTACTTATGCATCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1432
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CCATTAGAGACTGGCGCACA
Suggested primer 2:AGCGACCCCACCTAACTAGT