Insertion junction: CLIP2.046342_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.046342
Chromosome:chromosome 1
Location:4802716
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g033400 TIM9 Mitochondrial inner membrane translocase subunit; (1 of 1) K17777 - mitochondrial import inner membrane translocase subunit TIM9 (TIM9) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAGCGCATGCAGTTTAGGGCGCTCAACCCGTGCGTGCAGCCGGCGATGC
Internal bar code:CTCGACCGCTTAGGGATTCTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1928
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCGCTGATGATGTCATTT
Suggested primer 2:CGTGGCATTTGGGTGTTCTG