| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.046343 |
| Chromosome: | chromosome 4 |
| Location: | 3563611 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g228400 | WRK1 | (1 of 1) PF03106 - WRKY DNA -binding domain (WRKY); WRKY family transcription factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTCGCACAATTGCGTACTGTGACTGACTATGCGGGGCGTGGCCCCCCC |
| Internal bar code: | TTTTAGGGGGATTACGCCTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5316 |
| LEAP-Seq percent confirming: | 97.3684 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCTGTTCTGCTTCCACTA |
| Suggested primer 2: | TAGCGACAGCCTTATCAGCG |