Insertion junction: CLIP2.046369_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.046369
Chromosome:chromosome 10
Location:2237957
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g433800 CSE21 Predicted protein of CSE family CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAGTGCAGTTGTGTTCGGGCCATGGTCGCGATAGGCTTGTGGTAGGCTC
Internal bar code:TTAGGTTATAGTATTACAAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3616
LEAP-Seq percent confirming:93.2432
LEAP-Seq n confirming:69
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:74

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTTGACATGACTGGACCA
Suggested primer 2:TTCGCGACTGAGAAGCTTGT