| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.046378 |
| Chromosome: | chromosome 12 |
| Location: | 4239702 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g518107 | (1 of 1) PF04182 - B-block binding subunit of TFIIIC (B-block_TFIIIC) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGGGGCTAAGGGTGCTGGATATGCAAGGCTGCTTGGCGGCTTACGTA |
| Internal bar code: | CGGCGAGCCTAGATGTTTTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 492 |
| LEAP-Seq percent confirming: | 8.33333 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGATGGGTTCGGATTGGT |
| Suggested primer 2: | GCGCATGGCCAAGAAATGAA |