Insertion junction: CLIP2.046379_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.046379
Chromosome:chromosome 3
Location:7614098
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g204601 CPK3,FAP278 Flagellar Associated Protein 278; (1 of 1) K00856 - adenosine kinase (E2.7.1.20, ADK) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGTCCGTGTCATACGCACGCGGCCTGCCACGTGCCGCGTCAAACCCGC
Internal bar code:AACATCGCTGTTCTTGTAACGT

Confirmation - LEAP-Seq

LEAP-Seq distance:86
LEAP-Seq percent confirming:40.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CGATACCGGACTCCACAGTG
Suggested primer 2:CCGCCAACAACTTCAAGGTG