| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.046387 |
| Chromosome: | chromosome 6 |
| Location: | 3549467 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278123 | VPS39 | (1 of 781) IPR000104 - Antifreeze protein, type I; Subunit of VPS-C complex | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGCTGTTATGCTGGCGCTGGAGCACAAGGTGCTTGGTGGGCGAGGAGC |
| Internal bar code: | GTTGCTCCCAGAATAAAGAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1570 |
| LEAP-Seq percent confirming: | 58.3333 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCTAGCTGGCGAGTTCG |
| Suggested primer 2: | GTTGTTGTTGGTGGTGGTGG |