| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.046390 |
| Chromosome: | chromosome 2 |
| Location: | 8093229 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g144250 | CYP769A1,CYP13 | (1 of 1) 1.14.13.30 - Leukotriene-B(4) 20-monooxygenase / LTB(4) omega-hydroxylase; Cytochrome P450, CYP4 superfamily | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGTTACGAACGGGTGCCGCCCCAGCGCCCCCAAGAACAGGCACAGGCC |
| Internal bar code: | ACTATGCTCACATGGCGCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 970 |
| LEAP-Seq percent confirming: | 83.7838 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGGCTACGACTGTGTGT |
| Suggested primer 2: | CGGGAGGTTACATGCATGCT |