Insertion junction: CLIP2.046391_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.046391
Chromosome:chromosome 16
Location:7999385
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g692004 DNJ21 (1 of 8) PF00226//PF01556 - DnaJ domain (DnaJ) // DnaJ C terminal domain (DnaJ_C); DnaJ-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTCCTCTGTTTCCGCCCAGCTGCCCGTCGCCACATCGTGCCCCAGCCCA
Internal bar code:GTTGAATTTCGCAATGGATGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:956
LEAP-Seq percent confirming:28.8889
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:GTTCACCGGAGAAACGTTGC
Suggested primer 2:ATGATTGCTTGGACCCCCAG