Insertion junction: CLIP2.046392_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.046392
Chromosome:chromosome 6
Location:8047998
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g305050 EEP5,CGL45 expressed protein Conserved in the Green Lineage; (1 of 90) PF03372 - Endonuclease/Exonuclease/phosphatase family (Exo_endo_phos) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAATCTTTATATACCCAAGCCTGTTCACTCCGCGCCATCACACTCCTCTC
Internal bar code:GTTCGAGCAAAGTTTCCTTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3159
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:CACTCCAATTCAGGTCGCCT
Suggested primer 2:GACCTGGAACCTGTGGTGAG