Insertion junction: CLIP2.046393_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.046393
Chromosome:chromosome 12
Location:2193382
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g509150 (1 of 1) PTHR18937//PTHR18937:SF151 - STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCACGCAGCGCCCAGCACCTAGCCCACCTTGATGATCGCGTCATTGAGC
Internal bar code:CTCAACAAGATGTAGGTTTTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:4998
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:142
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:142

Suggested primers for confirmation by PCR

Suggested primer 1:TCTTGCGCTGATGTCCAACT
Suggested primer 2:CTGGCCCTAGCTCACTGATG