Insertion junction: CLIP2.046401_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.046401
Chromosome:chromosome 2
Location:6903536
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g388801 (1 of 1) IPR001615 - Delta-endotoxin CytB intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTCCAGAGTTTTGCTTTTGCGGCTGGCCGAAACAACTGCGTGTGACTT
Internal bar code:GTTGCGTGACCATGGGATGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1213
LEAP-Seq percent confirming:93.75
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GTAACATGGTGGCGCGAATC
Suggested primer 2:TGCGGCTCTCAAACAAAAGC