Insertion junction: CLIP2.046402_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.046402
Chromosome:chromosome 2
Location:804341
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g078550 FAP210 (1 of 5) PF13868 - Trichohyalin-plectin-homology domain (TPH); Flagellar Associated Protein 210 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCCCGTCCCCACGCCTTTGGCGCACACTCCGCACCCCGCAGCTCGACC
Internal bar code:CTTAACTAGTAATCATAGAACC

Confirmation - LEAP-Seq

LEAP-Seq distance:1889
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTCACCTCTACTACCCCA
Suggested primer 2:GCGCCTAAACGAATGCAGAG