| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.046412 |
| Chromosome: | chromosome 6 |
| Location: | 2359499 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g268000 | HFO10 | (1 of 33) K11254 - histone H4 (H4); Histone H4 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGACGTGGCAAGGGCGGCAAGGGCCTGGGCAAAGGCGGTGCCAAGCG |
| Internal bar code: | AGATAATGTGAGGGGTTCTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1354 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTTACCAGCCTGCAGACC |
| Suggested primer 2: | ACACCGAGTTTTGTTGGGGT |