Insertion junction: CLIP2.046440_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.046440
Chromosome:chromosome 1
Location:204750
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g001300 ankryin repeat containing protein; (1 of 1) IPR000782//IPR006626 - FAS1 domain // Parallel beta-helix repeat CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACAGGATGAGAAGCAGGTTCACGGCTGTGTGTGTGGGGGGGGAAGGGGG
Internal bar code:GAACTAGGGTGCGGCATCCGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:339
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GGAAGGAGAGGAGGGCTGTA
Suggested primer 2:GTAAGGTGGTGGGAACTGGG