Insertion junction: CLIP2.046440_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.046440
Chromosome:chromosome 1
Location:204754
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g001300 ankryin repeat containing protein; (1 of 1) IPR000782//IPR006626 - FAS1 domain // Parallel beta-helix repeat CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTTTGCCAACATGGACATTGGCGAGGACCCGGACGAGGCAAGCATCGG
Internal bar code:GAACTAGGGTGCGGCATCCGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:47
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTAAGGTGGTGGGAACTGGG
Suggested primer 2:GGAAGGAGAGGAGGGCTGTA