Insertion junction: CLIP2.046441_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.046441
Chromosome:chromosome 7
Location:992486
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g319452 PHC73 Putative pherophorin-chlamydomonas homolog; (1 of 2) IPR003072//IPR003882 - Orphan nuclear receptor, NOR1 type // Pistil-specific extensin-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTAAACAGTACTGTATACATTTGGGTGCCTGTCGTCGTCGGCGCCAAG
Internal bar code:TAGAATTCATGGTACGCCTTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1832
LEAP-Seq percent confirming:94.3662
LEAP-Seq n confirming:67
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:71

Suggested primers for confirmation by PCR

Suggested primer 1:TTCTGCTAGTCATTGCGGCA
Suggested primer 2:GTCCTGGTGGTGAGAGGTTG