Insertion junction: CLIP2.046442_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.046442
Chromosome:chromosome 7
Location:5856557
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g353750 MMP10 Metalloproteinase of VMP family; (1 of 40) 3.4.24.38 - Gametolysin / Lysin intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTGGTCTGCGCGCTGCGTTGCGACGGTTGTGCCAGGCGGTTTTACGAA
Internal bar code:ACCGGTTAAGTCACGGGGATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:159
LEAP-Seq percent confirming:45.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCCCATTCTTGTCCGCCT
Suggested primer 2:AAGTACTCAACTGCGCACGA