| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.046451 |
| Chromosome: | chromosome 14 |
| Location: | 1280552 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616350 | FAS4 | Fasciclin-like protein 4; (1 of 10) PTHR10900 - PERIOSTIN-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAGTGAGTGACTAGGTCACATTTCAGTGGCGGGTCGGGGGAGAGGGAA |
| Internal bar code: | ACTTCTAGATCAGTCGAACACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2134 |
| LEAP-Seq percent confirming: | 67.7632 |
| LEAP-Seq n confirming: | 103 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 152 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGGGATATGTAGTGCCG |
| Suggested primer 2: | CGTACGGACAGGGTTTTCGA |