| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.046453 |
| Chromosome: | chromosome 2 |
| Location: | 7357401 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391838 | (1 of 32) 3.1.1.3 - Triacylglycerol lipase / Triglyceride lipase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTGGGCACTGCACTACGCGTCACATTCAGCCCACGCGCACTATGTGT |
| Internal bar code: | CGCTATACCATTTTCTATCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3432 |
| LEAP-Seq percent confirming: | 37.5 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 112 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGTTTACCCTCCAGTCCC |
| Suggested primer 2: | ATTCACCTGCATCCCCAAGG |