Insertion junction: CLIP2.046455_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.046455
Chromosome:chromosome 1
Location:2339878
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCTATTAGCAACAAGTCAGCGAGTGTTCTGCTTGCGTGTAGATCCGTT
Internal bar code:AATTCTTTTTACTCTCTCGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:4636
LEAP-Seq percent confirming:84.2697
LEAP-Seq n confirming:75
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:89

Suggested primers for confirmation by PCR

Suggested primer 1:GCACAGTTGCTGATGCCAAA
Suggested primer 2:CAGGTGTGAAGTACGGCAGT