| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.046460 |
| Chromosome: | chromosome 10 |
| Location: | 3319446 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443300 | CNK5 | NimA-related protein kinase; (1 of 1) PTHR24361//PTHR24361:SF368 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCGGCTAAGGAGTACTTCACGGAGGACGAGGTCATGAACATGTTTGTG |
| Internal bar code: | TAAAATACCTTTCATATAGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1290 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGGCGCCATGTAGTAGG |
| Suggested primer 2: | CCCAACGAGATAGAGCGGAC |