| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.046483 |
| Chromosome: | chromosome 1 |
| Location: | 5950586 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g800105 | 5'UTR | ||
| Cre01.g800106 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCAACATTCGTAAATGCAACAATTATTGCTGATTTGGATGTTACGAAC |
| Internal bar code: | ATGCAAAACGGTATAGTCCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1104 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACTCCGCACCCACGATAG |
| Suggested primer 2: | CTCCGTCGCCTGTTCCTC |